levels of o....................

p........

t...........................

levels of m...........................

of work

2. To avoid contaminating the culture with

and accidently culturing them.

3. To avoid letting harmful micro-organisms

1)

work surfaces with chemicals.

2) Sterilising media (using,

121oC for 15-20 mins)

3) Sterilising equipment (using heat e.g. flaming inoculating loop & bottle tops in

).

3) Hands must be

thoroughly before and after, cuts covered and lab coat worn.

4) An appropriate medium e.g. agar or nutrient broths which vary in the pH and the nutrients they contain depending on what type of cells are to grow

5) Control of other factors such as temperature and oxygen