Quantification is nor possible without a calibration curve

Chromatographic separation depends on the partitioning of analytes between a stationary and a mobile phase

The effectiveness of a chromatographic separation depends only onthe mobile phase

Chromatography by itself is an identification technique useful for many types of compounds

Chromatography is only useful as a separation technique

The limit of detection will depend on the detector used

Compounds retained more strongly by the stationary phase elute at the beginning of the elution

An eluate is a fraction, collected at the exit of the column, that contains only one analyte

The eluate has a shorter retention time than any eluent

At the end of the elution the eluate contains only the eluent

Elution happens as the eluate carries analytes downstream

The eluent helps separates analytes in the elutes during elution

In SEC, molecules smaller or larger than a certanin range (the selective permeation region) will not be separated by size

Gel permeation Chromatography is another name for SEC

Large molecules elute last in SEC due to their larger volume

For SEC, the polarity of the stationary phase is an important factor

The pore size of the stationary phase is an important factor

Size exclusion Chromatography is only useful for macromolecules such as polymers and proteins

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