dilute concentration in (µg/ml) which is the protein concentration which was obtained from the graph,

the undiluted concentration which is the dilute concentrated multiplied by the dilution factor

C1/v1=c2/v2

This is identified when the colour of the assay changes to purple colour this colour is formed when the cu2+ ions and peptide bonds interact. This reaction takes place in alkaline conditions. The biuret reagent is placed into the protein solution. The amount of colour is proportional to the degree of peptide bonds present. The absorbance of the Cu2+-protein complex is measured at 540 nm

tyrosine and tryptophan in the protein with the biuret reaction and also the folin- ciocalteau phenol reagent to produce a bluish colour. these are formation of the bond between peptide bond nitrogen and also copper ion. The other includes reduction of the Folin-Ciocalteu reagent by tyrosine. This reaction has an absorption range of 650 -750nm using a

The main reaction that takes place in the BCA is the reaction which causes cu2+ to convert to cu1+, this takes place when in alkaline conditions, the reaction which takes place is called the biuret reaction, this reaction Is influenced by certain amino acids these are cysteine, tryptophan typosine with the addition of a peptide backbone.

20-2000 MICROGRAMS /ML

10-1000

20-2000 MICROGRAMS /ML