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Practical 4

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what is invertase made of ?

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what happens to invertase as temperature increases ? 0-40 ,50.60, 70-100

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the molecular collisions increase as the temperature increases.
substrate reaches maximum begins to deature
The temperature exhibits the same energy as the enzyme leading to an increase in reaction, and the glycosidic bonds break which is why we see a in crease in reaction

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How To Calculate Rate of reacting ( V) ?

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How to calculate Specific activity of invertase ?

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explain the 2 reactions that takes place in Practical 4

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Explanation

pH of invertase was 4.7 and the sodium hydroxide is higher pH so it inactivates the invertase stopping the reaction

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Reduction of sucrose to fructose and glucose

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Fisher projection or glucose and fructose

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what wavelength does ANS ABSORB AT?

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What group gets reduced in the DNS ? and what to ?

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What are the reducing groups on fructose and glucose?

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Explain the shape of the curve (i.e. why does the rate increase with temperature, peaks and then decreases. What happens at boiling?

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why is glucose alpha D ?

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