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The most widely accepted peroxisome origin theory is that peroxisomes were derived from the

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A good piece of evidence for this theory is that genetic peroxisome-less mutants of yeast given the wild type gene will

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Another good piece of evidence is certain peroxisomal proteins are

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Peroxisomes were thought to originate from an environment where the oxygen levels were

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For many organisms at this time oxygen was

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So peroxisomes developed to help organisms detoxify

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What is the size range of a peroxisome (no units are needed, presume all answers are in µm)?

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2-10

0.1-2

0.1-1.5

0.05-2

3-5

0.1 to 1

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Identify all the common features present in all peroxisomes:

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Organelle division machinery

Fatty acid metabolism and transport

Glyoxylate cycle

Catalase

Photorespiratory enzymes

ROS detoxification detoxification

Protein import machinery

Plasmalogen generation machinery

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Peroxisomes are single

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A region of higher electron density may or may not be present, this is called a

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Enzymes which are localised to this region are ..

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The inner region of the peroxisome is referred to as

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A lot of what are present within this region

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Match the name of the peroxin with it's function:

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Match the text
Pex7p
Pex5p
Pex12p, Pex10p and Pex2p
Pex8p
Pex17p, Pex14p and Pex13p
Pex12p
Pex20p and Pex7p
Pex18p, Pex7p and Pex21p

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Recognises PTS-2
One of 3 ubiquitin ligases.
Acts as a connection bridge between Pex12p and Pex13p
Recognises PTS-1
Recognises PTS-2 in most fungi
Form a ring complex in the plasma membrane
Recognises PTS-2 in yeast
Form the peroxisomal docking complex

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How many PEX genes have been identified in yeast?

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50

40

60

10

100

30

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Match the text
Arabidopsis thaliana
Homo sapien
Rattus norvegicus
Saccharomyces cerivisiae

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Animal
Fungi
Animal
Plants

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Glyoxysome
Leaf peroxisome
Root nodule peroxisome
Undifferentiated peroxisome

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Contain mainly catalase and uricase.
Involved in glycol ate metabolism and photorespiration,
Involved in the biosynthesis of allantoin
Important in germination and growth, combines two processes to make sugars available.

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What enzymes may be localised to the crystalline/crystalloid core?

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Urate oxidases

Xanthine oxidases

Catalase

Uric acid oxidase

Oxidase enxymes

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What is the range of number of peroxisomes which can be found in a single cell?

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50-200

100-1000

1000-10000

10-100

1-100000

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At least how many different peroxins are involved in the mechanism of protein import into the peroxisome?

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23

10

32

16

100

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Match the text
Marginal plate
Crystalline core
Matrix
Peroxisome membrane

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A single lipid bilayer
Made of oxidase, mainly rate oxidase
Contains many enzymes, frequently catalase
Made of L-alpha-hydroxyacid oxidase B

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What is the name given to peroxisome proteins?

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peroxisomal proteins

peroxins

Pex

peroxides

Peroxynes