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HISTO-FIXATION TROUBLESHOOTING

histo-fixation-troubleshooting

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block

Moth Eaten Appearance

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Sections crumble and tear out of block

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Tissue shrinkage when tissue attaches to glass slide, more pronounced in Formalin-fixed tissues

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Cracked blocks

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Chatter Artifact/Washboard appearance is due to ?

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Poor/absent staining of nuclei in H&E is most likely due to?

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How do you prevent sponge artifact?

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A "disk effect" occurs at the center next to the tissue

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Cloudy areas seen microscopically

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Crooked ribbons

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Ribbons fail to form

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Holes in sections

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Section lifts from knife as block raised

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Compressed/Wrinkled/Jammed sections

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Sections vary in thickness or skipped

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