Enzyme Kinetics

enzyme-kinetics-1

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Question 1 of 1

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451887

240

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Match the symbol equations with their meanings (E= enzyme, S = Substrate)

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Match the text
E + S → [ES]
[ES] → [ES*]
[ES*] → [EP]
[EP] → E + P

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Turnover of the Activated Subtrate to Product
Formation of the Michaelis-Menton Complex
Formation of the Activated Complex
Desorbing/Dissociation of product from the enzyme

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Rate of reaction (Vo) at a certain substrate concentration can be calculated by the Michaelis-Menten equation (differential rate equation) of:

Select the correct answer(s).

(0/0)

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What is meant by the term steady state kinetics?

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Initial rate (Vo) can be calculated by finding the gradient of the initial rate plot, where time (x) is plotted against substrate concentration (y) via:

Select the correct answer(s).

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451909

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Match the terms with their definition

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Match the text
Vmax
Km
kcat

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the number of reactions the active site catalyses per unit time (turnover number)
substrate concentration at which the rate is half-maximal
Maximum initial rate when when the enzyme is 100% saturated w

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What is the relationship between Vmax and Kcat?

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Vmax is a constant (fixed number).

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Explanation

Value of Vmax depends on the the enzyme Concentartion when it is 100% saturated (binded) with a substrate.

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If concentration of enzyme is doubled we should...

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Explanation

The concentration of substrate will also have to be considered

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The Michaelis Constant can also be written in the term of...

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Plotting initial rates (Vo) against a series of different substrate concentrations [S] but at a single, constant, enzyme concentration obtains a

Select the correct answer(s).

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451935

120

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In a hyperbolic/Saturation plot, reaction becomes non-linear after __ % or more of the substrate have been consumed.

Select the correct answer(s).

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In a hyperbolic/saturation plot, if we look at the graph at low substrate (Km > [S]), simplifying it with the Michaelis-Menton equation results in what type of order reaction with respect to [S]?

Select the correct answer(s).

(0/0)

451938

120

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In a hyperbolic/saturation plot, if we look at the graph at very high substrate (Km < [S]), simplifying it with the Michaelis-Menton equation results in what type of order reaction with respect to [S]?

Select the correct answer(s).

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451959

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How many points at minim do we need to cover the saturation curve to obtain an accurate answer?

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451984

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Enzyme Kinetics

Question 1 of 1

Time Left

Match the symbol equations with their meanings (E= enzyme, S = Substrate)

Match the text (click and drag)

(0/0)

E + S → [ES]

[ES] → [ES*]

[ES*] → [EP]

[EP] → E + P

Turnover of the Activated Subtrate to Product

Formation of the Michaelis-Menton Complex

Formation of the Activated Complex

Desorbing/Dissociation of product from the enzyme

Rate of reaction (Vo) at a certain substrate concentration can be calculated by the Michaelis-Menten equation (differential rate equation) of:

Select the correct answer(s).

(0/0)

What is meant by the term steady state kinetics?

Select the correct answer(s).

(0/0)

Initial rate (Vo) can be calculated by finding the gradient of the initial rate plot, where time (x) is plotted against substrate concentration (y) via:

Select the correct answer(s).

(0/0)

Match the terms with their definition

Match the text (click and drag)

(0/0)

Vmax

Km

kcat

the number of reactions the active site catalyses per unit time (turnover number)

substrate concentration at which the rate is half-maximal

Maximum initial rate when when the enzyme is 100% saturated w

What is the relationship between Vmax and Kcat?

Select the correct answer(s).

(0/0)

Vmax is a constant (fixed number).

Select the correct answer(s).

(0/0)

Explanation

If concentration of enzyme is doubled we should...

Select the correct answer(s).

(0/0)

Explanation

The Michaelis Constant can also be written in the term of...

Select the correct answer(s).

(0/0)

Plotting initial rates (Vo) against a series of different substrate concentrations [S] but at a single, constant, enzyme concentration obtains a

Select the correct answer(s).

(0/0)

In a hyperbolic/Saturation plot, reaction becomes non-linear after __ % or more of the substrate have been consumed.

Select the correct answer(s).

(0/0)

In a hyperbolic/saturation plot, if we look at the graph at low substrate (Km > [S]), simplifying it with the Michaelis-Menton equation results in what type of order reaction with respect to [S]?

Select the correct answer(s).

(0/0)

In a hyperbolic/saturation plot, if we look at the graph at very high substrate (Km < [S]), simplifying it with the Michaelis-Menton equation results in what type of order reaction with respect to [S]?

Select the correct answer(s).

(0/0)

How many points at minim do we need to cover the saturation curve to obtain an accurate answer?

Select the correct answer(s).

(0/0)

What is the Substrate Range ( i.e. difference between the lowest and highest concentrations of [substrate] we should use?

Select the correct answer(s).

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