BioProcessing and Other shit

cell-characterisation-purification-and-harvesting

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Question 1 of 1

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463471

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What methods can be used to identify cell characteristics (Select one with 4 correct methods)

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463473

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How many times do you wash antibodies when using secondary and primary, for fluorescent antigen labelling?

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463474

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Are the following cell characterisation methods destructive: 1) Gene Expression Profiles, 2) Phosphate Profiling, 3) Global Proteomic Profiling, 4) Cell Surface Proteome Profile, 5) Functional Assays

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Explanation

Destructive Cell Characterisation Methods Are: Gene Expression Profiling Phosphorate Profiling Global Proteomic Profling Cell Surface Proteomic Profile Functional Assays Non-Destructive Cell Characterisation Methods Are: Secreted Proteins Cell Surface Antigens

463476

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What was wrong with Solohills Harvesting Approach

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463480

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If I want to put to have a Midbrain Neuron, when should and what growth factors should I add?

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463485

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what cell characteristics can be looked at for cell stage identification?

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463487

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What is the Oxygen Tension in a normal lab? Is this good for Cell Expansion?

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463493

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What step did the novel harvesting procedure add?

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463499

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Match The CQA to appropriate definition

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Match the text
Safety
Potency
Purity
Identity

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To ensure media transplanted does not contain any unidentified culture culture media, and in the case of stem-cell derived product undifferentiated cells that may cause harm.
To ensure that the biological product being transplanted is of the appropriate phenotype to carry out the desired mode of action
To ensure transplanted media is compatible to recipient, is free from any contaminating microbes and does not carry any genetic instability
To ensure that the cell therapy achieves the appropriate level of clinical efficacy

463501

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Gene Expression Profiling Proteomic Profiling Epigenetic Profiling Antibody Targeting Methods Immunofluorescent Labelling and Microscopy Metabolic Profiling What CQA does the above asses?

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463505

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Quality Assurance refers to the constant assessment of the cell product. Is this true?

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Explanation

Quality Assurance: Refers to the process used to create the product, as well as alternative methods to ensure product quality, such as staff training. Key Word Here is: Process Quality Control refers to a set of measures implicated by the manufacturer at various points throughout the process to ensure that the Product is of an acceptable standard (e.g. Inspection, Potency Testing)

463507

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? is difficult to assess due to the unknown MOAs of multiple cell therapies (i.e. Cardiac Therapies) What is the ? mark referring to.

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463766

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Which of these is not a bioprocessing parameter: Morphology PH and C20 Bioprocessing Environment (Biological Safety Cabinet or Incubator) Oxygen Tension Duration of Culture Temperature

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463768

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We can only use scale up processes for Autologous Cell Therapies. Is this statement true?

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Explanation

Autologous = Scale Out Allogenic = Scale Up

463513

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Which cells are most difficult to work with

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Explanation

ESCs and ISPCs are both pluripotent stem cells, they are difficult to work with, but individually one is not necessarily more difficult to work with then the other.

BioProcessing and Other shit

Question 1 of 1

Time Left

What methods can be used to identify cell characteristics (Select one with 4 correct methods)

Select the correct answer(s).

(0/0)

How many times do you wash antibodies when using secondary and primary, for fluorescent antigen labelling?

Select the correct answer(s).

(0/0)

Are the following cell characterisation methods destructive: 1) Gene Expression Profiles, 2) Phosphate Profiling, 3) Global Proteomic Profiling, 4) Cell Surface Proteome Profile, 5) Functional Assays

Select the correct answer(s).

(0/0)

Explanation

What was wrong with Solohills Harvesting Approach

Select the correct answer(s).

(0/0)

If I want to put to have a Midbrain Neuron, when should and what growth factors should I add?

Select the correct answer(s).

(0/0)

what cell characteristics can be looked at for cell stage identification?

Select the correct answer(s).

(0/0)

What is the Oxygen Tension in a normal lab? Is this good for Cell Expansion?

Select the correct answer(s).

(0/0)

What step did the novel harvesting procedure add?

Select the correct answer(s).

(0/0)

Match The CQA to appropriate definition

Match the text (click and drag)

(0/0)

Safety

Potency

Purity

Identity

To ensure media transplanted does not contain any unidentified culture culture media, and in the case of stem-cell derived product undifferentiated cells that may cause harm.

To ensure that the biological product being transplanted is of the appropriate phenotype to carry out the desired mode of action

To ensure transplanted media is compatible to recipient, is free from any contaminating microbes and does not carry any genetic instability

To ensure that the cell therapy achieves the appropriate level of clinical efficacy

Gene Expression Profiling Proteomic Profiling Epigenetic Profiling Antibody Targeting Methods Immunofluorescent Labelling and Microscopy Metabolic Profiling What CQA does the above asses?

Select the correct answer(s).

(0/0)

Quality Assurance refers to the constant assessment of the cell product. Is this true?

Select the correct answer(s).

(0/0)

Explanation

? is difficult to assess due to the unknown MOAs of multiple cell therapies (i.e. Cardiac Therapies) What is the ? mark referring to.

Select the correct answer(s).

(0/0)

Which of these is not a bioprocessing parameter: Morphology PH and C20 Bioprocessing Environment (Biological Safety Cabinet or Incubator) Oxygen Tension Duration of Culture Temperature

Select the correct answer(s).

(0/0)

We can only use scale up processes for Autologous Cell Therapies. Is this statement true?

Select the correct answer(s).

(0/0)

Explanation

Which cells are most difficult to work with

Select the correct answer(s).

(0/0)

Explanation

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