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CAR-T Cell Therapies

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What are the different types of T-Cells

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What is CAR

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What is the first step of CAR-T Cell Therapy Manufacturing

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Are T-Cell therapies Allogenic or Autologous

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What are the Manufacturing Challenges of Leukapheresis?

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What is the way to detach Dynabeeds?

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What is a challenge of T-Cell Isolation?

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What is the superset cell type, of T-Cells

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What cell type needs to be Isolated after Leukapheresis

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Explanation

Leukapheresis = Sampling Blood, Need to isolate CD3 Complex T-Cells as they have a variable TCR Receptor, allowing for modification. Isolation can occur via: Magnetic Flow Cytometry, Counter Flow Centrifual Elutriation

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Antibodies (Dynabeeds) are added twice in the cell culturing process of CAR-T Cell Therapy.

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Explanation

Antibody Coated Beeds added once for Activation of T-Cells, before T-Cell Transduction (adding CAR infused Lent-viral Factor ) as this allows for CAR to be infused. Second they need to be added again to allow for Cell Proliferation (Modified CAR-T Cell Expansion)

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What is a risk of CAR-T Cell Therapy

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How do you remove Dynabeeds

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Explanation

In the cell culture, you must add DetachaBeeds which bind with Dynabeed Antibodies, after this magnetic flow cytometry allows for removal of Dynabeeds.

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Why do we use CAR-T Cell therapy?

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