CLINICAL CHEMISTRY-IRON

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Measured by adding sufficient Fe3+ to saturate all the binding sites on Transferrin, Removing the excess with MgCO3, and measuring the remaining iron

Measured by adding a known excess concentration of iron at neutral pH with a chromagen. The iron that didn't bind Transferrin is free and binds the chromagen. Measurement is determined by the difference between the amount of iron added and the excess unbound iron present.

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