Extracting DNA

Sortie

Question 1 of 1

  Temps Restant


0
216374
0
30
block

How do you extract DNA?

Correspondre au texte (cliquer et faire glisser)   

(0/0)




Match le texte

1

2

3

4

5

6

7

Cliquer et faire glisser

Add ice cold ethanol (to prevent fire) to split the solution into layers based upon their solubility in ethanol.

Put the onion in a beaker with a solution of detergent and salt, the detergent breaks down the cell membrane as it is protein and the salt makes the DNA stick together.

Put the beaker in ice to cool the mixture down and prevent the DNA from breaking down.

Chop up onion.

Put the beaker into a water bath at 60c for 15 minutes to denature the enzymes that could digest the DNA.

Put the mixture into the blender to break open the cell wall and release the DNA.

The insoluble DNA will come out of the solution as a stringy white substance that can be collected with a glass rod.